![]() Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed. Store lipoparticles at -80 C or proceed to downstream assays Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation MixĤ. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagentģ. Clone your gene of interest using a TOPO™ vectorĢ. Arian Edalat PhD, who ran the MembranePRO business since 2011 as VP of Engineering at Water Planet, has been named president and general manager of the new company, which continues to operate under the MembranePRO Services name. With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:ġ. Los Angeles, California-based Water Planet has divested its MembranePRO Services business to a group of private investors. ![]() Lipoparticles are enriched with expressed receptors.Proteins bud off as lipoparticles and are easily collected from the culture medium.Cellular quality control and mammalian post-translational processing helps produce functional protein. ![]() Proteins are displayed on human (or other mammalian) cell membranes.In addition, benefits of MembranePro™ technology include: Easy to use-no enzymatic or mechanical dissociation required.Scalable-easy scale-up without the need for multiple flasks.Efficient-more than 20-fold increase in membrane protein yield compared to the adherent culture system.Hydranautics Nitto RO Membranes PRO-Series High Rejection PRO-XR. I've been dating this guy for 8 month, thinking he's my soul mate, only to be greatly dissapointed, heart broken and humiliated. RO Membranes PRO-Series Extra Ion Selectivity Nanofiltration PRO-XS. said 'Hiring these guys is Probably the best decision I've made in recent years. For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.įeatures of the Expi293™ MembranePro™ Expression System include: Emergency Services Water Treatment Equipment Installation Services Upgrades On-Site Water Systems Repair Hands-On Water System Training Water Equipment Leasing.
0 Comments
Leave a Reply. |